Hotspot

trnL-UAA-trnF-GAA

LSC · 49639-50042 · 404 bp

Back to Halerpestes

Pi 0.0873

MarkerSeek score 83.20

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 33
pi: 0.0873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	477-494 bp	1.0	85.2
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	CACATGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	495-512 bp	1.0	84.2
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	ATTAGCCACATGCCAGGAAC	50.0	50.0	57.5	58.2	501-518 bp	1.0	83.5
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	439-456 bp	1.0	82.5
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	474-491 bp	1.0	82.0