Hotspot

psbZ-trnG-GCC

LSC · 37666-38110 · 445 bp

Back to Halerpestes

Pi 0.115

MarkerSeek score 83.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 44
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGGTACATCATTATGGATTGGA	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	497-557 bp	1.0	64.6
psbZ-trnG-GCC_p2	TCCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	43.5	50.0	58.1	57.8	498-558 bp	1.0	64.6
psbZ-trnG-GCC_p3	CCGGTACATCATTATGGATTGG	CGTCTTCTCCTTGGCAAAGA	45.5	50.0	56.7	57.8	497-557 bp	1.0	62.9
psbZ-trnG-GCC_p4	CCGGTACATCATTATGGATTGGA	TACCATTCGACCATATCCGC	43.5	50.0	58.1	57.3	470-530 bp	1.0	60.4
psbZ-trnG-GCC_p5	TCCGGTACATCATTATGGATTGG	TACCATTCGACCATATCCGC	43.5	50.0	58.1	57.3	471-531 bp	1.0	60.4