Hotspot

trnD-GUC-trnY-GUA

LSC · 31553-31990 · 438 bp

Back to Halerpestes

Pi 0.141

MarkerSeek score 84.80

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 55
pi: 0.141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	TATTAGGTCGGGACTGACGG	TGGCAATATGTCTACGCTGG	55.0	50.0	58.3	57.8	533-560 bp	1.0	87.6
trnD-GUC-trnY-GUA_p2	TATTAGGTCGGGACTGACGG	GGCAATATGTCTACGCTGGT	55.0	50.0	58.3	57.8	532-559 bp	1.0	87.5
trnD-GUC-trnY-GUA_p3	TATTAGGTCGGGACTGACGG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.3	58.4	554-581 bp	1.0	86.4
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	481-508 bp	1.0	86.3
trnD-GUC-trnY-GUA_p5	TATTAGGTCGGGACTGACGG	GGGACGGACTGTAAATTCGT	55.0	50.0	58.3	57.6	553-580 bp	1.0	86.2