Hotspot

trnC-GCA-petN

LSC · 28579-29460 · 882 bp

Back to Halerpestes

Pi 0.125

MarkerSeek score 87.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 106
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	945-962 bp	1.0	90.1
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	939-956 bp	1.0	88.6
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	963-980 bp	1.0	87.8
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.4	57.8	957-974 bp	1.0	86.4
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CATTAAAGCAGCCCAAGCAA	50.0	45.0	58.1	57.3	947-964 bp	1.0	84.1