Hotspot

rpoB-trnC-GCA

LSC · 27465-28507 · 1043 bp

Back to Halerpestes

Pi 0.11

MarkerSeek score 82.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1043
variable_sites: 102
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCCGCCAATCCTTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1123-1205 bp	1.0	90.3
rpoB-trnC-GCA_p2	CCGGGGCTTTGCAATATTTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1415-1497 bp	1.0	89.6
rpoB-trnC-GCA_p3	GAAAATACCGGATCCCCACC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1742-1824 bp	1.0	88.9
rpoB-trnC-GCA_p4	CCCCACCTACACAAGCAAAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1729-1811 bp	1.0	88.6
rpoB-trnC-GCA_p5	AAATTAATCCCGCGGACACA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.9	58.1	1367 bp	0.5	58.4