Hotspot

rps16-trnQ-UUG

LSC · 6150-7482 · 1333 bp

Back to Halerpestes

Pi 0.206
MarkerSeek score 83.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1333
variable_sites
219
pi
0.206
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1206-1398 bp 1.0 85.1
rps16-trnQ-UUG_p2 CGTTGCTTTCTACCACATCG CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.2 57.8 1210-1402 bp 1.0 84.0
rps16-trnQ-UUG_p3 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1209-1401 bp 1.0 82.9
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG ACTCGGAGGTTCGAATCCTT 50.0 50.0 57.2 58.4 1211-1403 bp 1.0 82.2
rps16-trnQ-UUG_p5 GCACGTTGCTTTCTACCACA CTCGGAGGTTCGAATCCTTC 50.0 55.0 59.1 57.8 1213-1405 bp 1.0 81.8