Hotspot

rps15-ycf1

SSC · 122521-127472 · 4952 bp

Back to Halenia

Pi 0.0186
MarkerSeek score 48.90
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
4952
variable_sites
92
pi
0.0186
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ACGGTATGGATTTGACTGATGA TTATGCGCGGCTGGAAAT 40.9 50.0 57.2 57.8 170 bp 1.0 42.9
rps15-ycf1_p2 AACGGTATGGATTTGACTGATGA TTATGCGCGGCTGGAAAT 39.1 50.0 57.9 57.8 171 bp 1.0 42.8
rps15-ycf1_p3 ACGGTATGGATTTGACTGATG TTATGCGCGGCTGGAAAT 42.9 50.0 55.8 57.8 170 bp 1.0 42.8
rps15-ycf1_p4 ACGGTATGGATTTGACTGATGAG TTATGCGCGGCTGGAAAT 43.5 50.0 58.3 57.8 170 bp 1.0 42.8
rps15-ycf1_p5 CGGTATGGATTTGACTGATGAGT TTATGCGCGGCTGGAAAT 43.5 50.0 58.3 57.8 169 bp 1.0 42.8