Hotspot

ndhH-rps15

SSC · 122127-122256 · 130 bp

Back to Halenia

Pi 0.0234

MarkerSeek score 63.40

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 130
variable_sites: 3
pi: 0.0234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	GATGAGCCGAAGAACACCAT	CGGCTGTTGGCTTATTTGTC	50.0	50.0	58.0	57.7	305-307 bp	1.0	79.0
ndhH-rps15_p2	AACACCATGCATTGATGGGT	CGGCTGTTGGCTTATTTGTC	45.0	50.0	58.1	57.7	293-295 bp	1.0	78.8
ndhH-rps15_p3	AAGGATGAGCCGAAGAACAC	CGGCTGTTGGCTTATTTGTC	50.0	50.0	57.9	57.7	308-310 bp	1.0	78.4
ndhH-rps15_p4	TTTTCTTGTAGCCGGACCAG	CGGCTGTTGGCTTATTTGTC	50.0	50.0	57.8	57.7	242-244 bp	1.0	78.1
ndhH-rps15_p5	GATGAGCCGAAGAACACCAT	ACGGCTGTTGGCTTATTTGT	50.0	45.0	58.0	58.4	306-308 bp	1.0	78.0