Hotspot

ndhD-psaC

SSC · 116052-116140 · 89 bp

Back to Halenia

Pi 0.0562

MarkerSeek score 70.60

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 89
variable_sites: 5
pi: 0.0562
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCCGCCGATATTGGAAAA	ATGGCATGAAACAACTCGCA	45.0	45.0	57.9	58.8	211-212 bp	1.0	74.8
ndhD-psaC_p2	ACCCGCCGATATTGGAAAAA	ATGGCATGAAACAACTCGCA	45.0	45.0	57.9	58.8	210-211 bp	1.0	74.8
ndhD-psaC_p3	AAAATTAAGGAACCCGCCGA	ATGGCATGAAACAACTCGCA	45.0	45.0	57.8	58.8	221-222 bp	1.0	74.2
ndhD-psaC_p4	GAACCCGCCGATATTGGAAA	ATGGCATGAAACAACTCGCA	50.0	45.0	58.3	58.8	212-213 bp	1.0	73.2
ndhD-psaC_p5	TCCTATTTCCCCTGTGTGGA	ATGGCATGAAACAACTCGCA	50.0	45.0	57.6	58.8	247-248 bp	1.0	72.8