Hotspot

ndhF-rpl32

SSC · 111230-111983 · 754 bp

Back to Halenia

Pi 0.0193
MarkerSeek score 61.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
754
variable_sites
14
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACGGGAAGTGGAATGAA TGCTGTAGAAATGCGCTTTT 50.0 40.0 57.8 56.6 833-843 bp 1.0 71.5
ndhF-rpl32_p2 GGAACGGGAAGTGGAATGAA TTGCTGTAGAAATGCGCTTT 50.0 40.0 57.8 56.6 834-844 bp 1.0 71.5
ndhF-rpl32_p3 GGAACGGGAAGTGGAATGAA TTTGCTGTAGAAATGCGCTT 50.0 40.0 57.8 56.6 835-845 bp 1.0 71.5
ndhF-rpl32_p4 GGAACGGGAAGTGGAATGAA TTTTGCTGTAGAAATGCGCT 50.0 40.0 57.8 56.6 836-846 bp 1.0 71.5
ndhF-rpl32_p5 CGGGAAGTGGAATGAAAGGT TGCTGTAGAAATGCGCTTTT 50.0 40.0 57.8 56.6 829-839 bp 1.0 71.5