Hotspot

psbT-psbN

LSC · 73940-73999 · 60 bp

Back to Halenia

Pi 0.0333

MarkerSeek score 70.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 2
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	CCATTTGAGGAACATGGGGA	50.0	50.0	57.6	57.5	124 bp	1.0	74.2
psbT-psbN_p2	TCGAGAACCGCCTAAAGTTC	TCCATTTGAGGAACATGGGG	50.0	50.0	57.6	57.5	125 bp	1.0	74.2
psbT-psbN_p3	GAGAACCGCCTAAAGTTCCA	CCATTTGAGGAACATGGGGA	50.0	50.0	57.5	57.5	122 bp	1.0	73.3
psbT-psbN_p4	GAGAACCGCCTAAAGTTCCA	TCCATTTGAGGAACATGGGG	50.0	50.0	57.5	57.5	123 bp	1.0	73.3
psbT-psbN_p5	CGAGAACCGCCTAAAGTTCC	CCATTTGAGGAACATGGGGA	55.0	50.0	58.6	57.5	123 bp	1.0	71.9