Hotspot

ycf3-trnS-GGA

LSC · 44676-45181 · 506 bp

Back to Halenia

Pi 0.0179

MarkerSeek score 64.10

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 9
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	666-669 bp	1.0	68.0
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	667-670 bp	1.0	68.0
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	637-640 bp	1.0	67.3
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	638-641 bp	1.0	67.3
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	ACACAAACCGGAAAGAGAGG	45.0	50.0	56.4	57.7	694-697 bp	1.0	66.7