Hotspot

trnG-GCC-trnfM-CAU

LSC · 36442-36678 · 237 bp

Back to Halenia

Pi 0.0306

MarkerSeek score 65.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 237
variable_sites: 7
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	303-305 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	304-306 bp	1.0	83.2
trnG-GCC-trnfM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	297-299 bp	1.0	82.7
trnG-GCC-trnfM-CAU_p4	GAGAAGATGCGGGTTCGATT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	298-300 bp	1.0	82.7
trnG-GCC-trnfM-CAU_p5	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.9	58.0	344-346 bp	1.0	82.4