Hotspot

petN-psbM

LSC · 28231-29321 · 1091 bp

Back to Halenia

Pi 0.0129

MarkerSeek score 60.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 14
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAGTCTTGCTTGGGCTG	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.8	58.0	1238-1248 bp	1.0	71.3
petN-psbM_p2	TCTTGCTTGGGCTGGTTTAA	TCATTCTAGTTCCTACCGCCT	45.0	47.6	57.6	58.0	1232-1242 bp	1.0	69.8
petN-psbM_p3	GGGGGCATAATTCACATGGA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.6	58.0	1261-1271 bp	1.0	69.7
petN-psbM_p4	GTCTTGCTTGGGCTGGTTTA	TCATTCTAGTTCCTACCGCCT	50.0	47.6	58.4	58.0	1233-1243 bp	1.0	69.7
petN-psbM_p5	AGGGGGCATAATTCACATGG	TCATTCTAGTTCCTACCGCCT	50.0	47.6	57.6	58.0	1262-1272 bp	1.0	69.7