Hotspot

rps16-trnQ-UUG

LSC · 5215-6542 · 1328 bp

Back to Halenia

Pi 0.0205
MarkerSeek score 62.50
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1328
variable_sites
27
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GGTTGAGCTCCCCTTTCAAG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.5 58.1 1406-1621 bp 1.0 78.1
rps16-trnQ-UUG_p2 GTTGAGCTCCCCTTTCAAGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.5 58.1 1405-1620 bp 1.0 78.1
rps16-trnQ-UUG_p3 ATATCCTGAACCGTTCCCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.5 58.1 1427-1642 bp 1.0 77.7
rps16-trnQ-UUG_p4 AATATCCTGAACCGTTCCCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1428-1643 bp 1.0 76.8
rps16-trnQ-UUG_p5 ACAGGTGCAACGATTAACGA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1373 bp 0.5 58.1