Hotspot

trnH-GUG-psbA

LSC · 75-416 · 342 bp

Back to Halenia

Pi 0.083

MarkerSeek score 71.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 19
pi: 0.083
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	315-416 bp	1.0	86.6
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	316-417 bp	1.0	86.3
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	308-409 bp	1.0	85.1
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TCCTCTAGACCTAGCTGCTG	50.0	55.0	57.9	57.6	316-417 bp	1.0	84.2
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TCCTCTAGACCTAGCTGCTG	50.0	55.0	58.2	57.6	317-418 bp	1.0	84.0