Hotspot

ycf1

SSC · 124485-129929 · 5445 bp

Back to Haemanthus

Pi 0.0103

MarkerSeek score 45.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5445
variable_sites: 84
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAGGTCGTTTTCGGGAAGAC	CCAAAATTGGTTAACAGACGGT	50.0	40.9	58.1	57.4	197-206 bp	1.0	62.0
ycf1_p2	GAAGGTCGTTTTCGGGAAGA	CCAAAATTGGTTAACAGACGGT	50.0	40.9	57.8	57.4	198-207 bp	1.0	61.7
ycf1_p3	AGAAGGTCGTTTTCGGGAAG	CCAAAATTGGTTAACAGACGGT	50.0	40.9	57.8	57.4	199-208 bp	1.0	61.6
ycf1_p4	AAAGAAGGTCGTTTTCGGGA	CCAAAATTGGTTAACAGACGGT	45.0	40.9	57.4	57.4	201-210 bp	1.0	57.9
ycf1_p5	AAGAAGGTCGTTTTCGGGAA	CCAAAATTGGTTAACAGACGGT	45.0	40.9	57.4	57.4	200-209 bp	1.0	57.9