Hotspot

ndhB

IRa · 142205-144436 · 2232 bp

Back to Haemanthus

Pi 0.000896
MarkerSeek score 57.80
Primer available yes
Label ndhB

Feature Metadata

label_name
ndhB
feature_type
gene
region
IRa
length_bp
2232
variable_sites
3
pi
0.000896
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB_p1 ATAACCCCAGCCCATTTTGG TTCGGAGATTGGATGCAGTT 50.0 45.0 58.1 57.2 2352 bp 1.0 72.8
ndhB_p2 ATAACCCCAGCCCATTTTGG TTTCGGAGATTGGATGCAGT 50.0 45.0 58.1 57.2 2353 bp 1.0 72.8
ndhB_p3 GTTCATAACCCCAGCCCATT TTCGGAGATTGGATGCAGTT 50.0 45.0 57.8 57.2 2356 bp 1.0 72.5
ndhB_p4 GTTCATAACCCCAGCCCATT TTTCGGAGATTGGATGCAGT 50.0 45.0 57.8 57.2 2357 bp 1.0 72.5
ndhB_p5 TTTGGGCGGAACAGATCTAC TTCGGAGATTGGATGCAGTT 50.0 45.0 57.6 57.2 2337 bp 1.0 70.6
ndhB_p1 TTCGGAGATTGGATGCAGTT ATAACCCCAGCCCATTTTGG 45.0 50.0 57.2 58.1 2352 bp 1.0 72.8
ndhB_p2 TTTCGGAGATTGGATGCAGT ATAACCCCAGCCCATTTTGG 45.0 50.0 57.2 58.1 2353 bp 1.0 72.8
ndhB_p3 TTCGGAGATTGGATGCAGTT GTTCATAACCCCAGCCCATT 45.0 50.0 57.2 57.8 2356 bp 1.0 72.5
ndhB_p4 TTTCGGAGATTGGATGCAGT GTTCATAACCCCAGCCCATT 45.0 50.0 57.2 57.8 2357 bp 1.0 72.5
ndhB_p5 TTCGGAGATTGGATGCAGTT TTTGGGCGGAACAGATCTAC 45.0 50.0 57.2 57.6 2337 bp 1.0 70.6