Hotspot

ndhI-ndhA

SSC · 121229-121316 · 88 bp

Back to Haemanthus

Pi 0.0455

MarkerSeek score 68.70

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 88
variable_sites: 6
pi: 0.0455
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	GCTCGTATTGTTTGTTGGCC	ACTTCTTCCCAACTTGTTTCAC	50.0	40.9	58.0	57.0	168-171 bp	1.0	60.2
ndhI-ndhA_p2	ACCTTGCAGCTCGTATTGTT	ACTTCTTCCCAACTTGTTTCAC	45.0	40.9	57.8	57.0	176-179 bp	1.0	58.7
ndhI-ndhA_p3	GCTCGTATTGTTTGTTGGCC	CTTCTTCCCAACTTGTTTCACT	50.0	40.9	58.0	56.7	167-170 bp	1.0	58.1
ndhI-ndhA_p4	GCTCGTATTGTTTGTTGGCC	TCTTCCCAACTTGTTTCACT	50.0	40.0	58.0	54.7	165-168 bp	1.0	57.8
ndhI-ndhA_p5	CCCGGTCACCATAATAGGGA	ACTTCTTCCCAACTTGTTTCAC	55.0	40.9	58.3	57.0	139 bp	0.333333	27.3