Hotspot

rpl32-trnL-UAG

SSC · 115212-115758 · 547 bp

Back to Haemanthus

Pi 0.014

MarkerSeek score 57.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 547
variable_sites: 11
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGCGGAACTGATTGATTGGA	AGGGCAGGCACTAATATTGC	45.0	50.0	57.5	58.0	1716-1883 bp	1.0	81.1
rpl32-trnL-UAG_p2	TGCGGAACTGATTGATTGGA	TAGCGAGCATTCCCTTTTCC	45.0	50.0	57.5	58.0	1466-1633 bp	1.0	81.0
rpl32-trnL-UAG_p3	TGCGGAACTGATTGATTGGA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.5	57.9	1205-1372 bp	1.0	80.5
rpl32-trnL-UAG_p4	TGCGGAACTGATTGATTGGA	AGATTGTAGGGCAGGCACTA	45.0	50.0	57.5	58.1	1723-1890 bp	1.0	80.3
rpl32-trnL-UAG_p5	TGCGGAACTGATTGATTGGA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.5	57.8	1198-1365 bp	1.0	80.0