Hotspot

trnN-GUU-rpl32

IRb · 110132-115038 · 4907 bp

Back to Haemanthus

Pi 0.00996

MarkerSeek score 42.50

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: IRb
length_bp: 4907
variable_sites: 46
pi: 0.00996
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AGCTTTCGAATAGGCATGGG	CCAATTGCGGCAAGTACTTG	50.0	50.0	58.0	58.0	563 bp	0.333333	53.0
trnN-GUU-rpl32_p2	AGCTTTCGAATAGGCATGGG	GGGATTTGTTCGGATCGGAA	50.0	50.0	58.0	58.0	451 bp	0.333333	52.9
trnN-GUU-rpl32_p3	AGCTTTCGAATAGGCATGGG	TAGCTATGGTGTTTTCGGCC	50.0	50.0	58.0	58.0	843 bp	0.333333	52.9
trnN-GUU-rpl32_p4	TATTGCAATTCCCCGAGTGG	CCAATTGCGGCAAGTACTTG	50.0	50.0	57.9	58.0	1026 bp	0.333333	52.8
trnN-GUU-rpl32_p5	TATTGCAATTCCCCGAGTGG	GGGATTTGTTCGGATCGGAA	50.0	50.0	57.9	58.0	914 bp	0.333333	52.7