Hotspot

petG-trnW-CCA

LSC · 67110-67226 · 117 bp

Back to Haemanthus

Pi 0.0345

MarkerSeek score 61.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 6
pi: 0.0345
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	GTGCTCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	211-212 bp	1.0	82.6
petG-trnW-CCA_p2	GTGCTCAGTTGGACCTTTGA	CCCTGTCATAAGAAAGCGCT	50.0	50.0	58.0	58.0	225-226 bp	1.0	82.3
petG-trnW-CCA_p3	GTGCTCAGTTGGACCTTTGA	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.0	58.1	872-877 bp	1.0	80.9
petG-trnW-CCA_p4	GTGCTCAGTTGGACCTTTGA	CCCGATGTATGCAAGACACA	50.0	50.0	58.0	58.0	286-287 bp	0.666667	66.5
petG-trnW-CCA_p5	TCAGGTATTCTACGGTCCCC	AGCGCTCTTAGTTCAGTTCG	55.0	50.0	57.9	58.0	449-450 bp	0.666667	66.1