Hotspot

atpB-rbcL

LSC · 55364-56238 · 875 bp

Back to Haemanthus

Pi 0.0061

MarkerSeek score 64.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 8
pi: 0.0061
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	57.9	56.6	983-1008 bp	1.0	69.6
atpB-rbcL_p2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	50.0	45.0	57.5	56.6	984-1009 bp	1.0	66.7
atpB-rbcL_p3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	57.9	57.2	983-1008 bp	1.0	65.8
atpB-rbcL_p4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	1007-1032 bp	1.0	65.3
atpB-rbcL_p5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	50.0	45.0	58.7	56.6	946-971 bp	1.0	65.2