primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	14562	15031	532-554	1.000000	81.7
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	14562	15031	588-610	1.000000	80.8
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	14562	15031	533-555	1.000000	80.5
atpH-atpI_p4	atpH-atpI	4	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	14562	15031	592-614	1.000000	80.2
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.8	57.9	0.0	21.3	0.280185	14562	15031	547-569	1.000000	80.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	32425	33136	793-855	1.000000	84.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	32425	33136	772-834	1.000000	83.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	32425	33136	769-831	1.000000	83.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32425	33136	755-817	1.000000	83.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32425	33136	734-796	1.000000	83.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47618	48515	945-985	1.000000	82.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47618	48515	953-993	1.000000	81.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47618	48515	977-1017	1.000000	81.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47618	48515	985-1025	1.000000	80.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47618	48515	968-1008	1.000000	80.2
atpB-rbcL_p1	atpB-rbcL	1	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACA	20	20	45.0	45.0	57.9	56.6	2.1	0.0	1.507995	55364	56238	983-1008	1.000000	69.6
atpB-rbcL_p2	atpB-rbcL	2	CTGGGCCAATAATTTGAGCG	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	57.5	56.6	8.0	0.0	1.868803	55364	56238	984-1009	1.000000	66.7
atpB-rbcL_p3	atpB-rbcL	3	TGGGCCAATAATTTGAGCGA	AGTCTCTGTTTGTGGTGACAT	20	21	45.0	42.9	57.9	57.2	2.1	0.0	1.982079	55364	56238	983-1008	1.000000	65.8
atpB-rbcL_p4	atpB-rbcL	4	CGGGGGAAAAACAACATCCA	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	58.7	56.6	0.0	0.0	2.038520	55364	56238	1007-1032	1.000000	65.3
atpB-rbcL_p5	atpB-rbcL	5	TCTTCAAGTGTGGAAACCGC	AGTCTCTGTTTGTGGTGACA	20	20	50.0	45.0	58.7	56.6	0.0	0.0	2.066495	55364	56238	946-971	1.000000	65.2
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	57.8	58.3	0.0	0.0	0.467291	63978	64977	1135-1137	1.000000	78.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.583398	63978	64977	1131-1133	1.000000	77.1
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTGGC	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.585102	63978	64977	1134-1136	1.000000	77.1
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	CGGTTCATATTCCGGGTTGG	20	20	50.0	55.0	57.8	58.7	0.0	0.0	0.849653	63978	64977	1136-1138	1.000000	75.0
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTGGC	TATTCCGGGTTGGGTTCATC	20	20	50.0	50.0	57.8	57.3	0.0	0.0	0.876279	63978	64977	1129-1131	1.000000	74.8
petG-trnW-CCA_p1	petG-trnW-CCA	1	GTGCTCAGTTGGACCTTTGA	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.0	58.0	0.0	2.6	0.048709	67110	67226	211-212	1.000000	82.6
petG-trnW-CCA_p2	petG-trnW-CCA	2	GTGCTCAGTTGGACCTTTGA	CCCTGTCATAAGAAAGCGCT	20	20	50.0	50.0	58.0	58.0	0.0	2.6	0.062520	67110	67226	225-226	1.000000	82.3
petG-trnW-CCA_p3	petG-trnW-CCA	3	GTGCTCAGTTGGACCTTTGA	TAGACCCGCTAAAGACCCAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.091304	67110	67226	872-877	1.000000	80.9
petG-trnW-CCA_p4	petG-trnW-CCA	4	GTGCTCAGTTGGACCTTTGA	CCCGATGTATGCAAGACACA	20	20	50.0	50.0	58.0	58.0	0.0	6.9	0.055446	67110	67226	286-287	0.666667	66.5
petG-trnW-CCA_p5	petG-trnW-CCA	5	TCAGGTATTCTACGGTCCCC	AGCGCTCTTAGTTCAGTTCG	20	20	55.0	50.0	57.9	58.0	0.0	2.6	0.093715	67110	67226	449-450	0.666667	66.1
ndhB_p1	ndhB	1	ATAACCCCAGCCCATTTTGG	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.909262	95933	98164	2352-2352	1.000000	72.8
ndhB_p2	ndhB	2	ATAACCCCAGCCCATTTTGG	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.909262	95933	98164	2353-2353	1.000000	72.8
ndhB_p3	ndhB	3	GTTCATAACCCCAGCCCATT	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	95933	98164	2356-2356	1.000000	72.5
ndhB_p4	ndhB	4	GTTCATAACCCCAGCCCATT	TTTCGGAGATTGGATGCAGT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	0.946652	95933	98164	2357-2357	1.000000	72.5
ndhB_p5	ndhB	5	TTTGGGCGGAACAGATCTAC	TTCGGAGATTGGATGCAGTT	20	20	50.0	45.0	57.6	57.2	0.0	0.0	1.184063	95933	98164	2337-2337	1.000000	70.6
trnN-GUU-rpl32_p1	trnN-GUU-rpl32	1	AGCTTTCGAATAGGCATGGG	CCAATTGCGGCAAGTACTTG	20	20	50.0	50.0	58.0	58.0	16.5	27.9	0.045874	113405	113654	563-563	0.333333	53.0
trnN-GUU-rpl32_p2	trnN-GUU-rpl32	2	AGCTTTCGAATAGGCATGGG	GGGATTTGTTCGGATCGGAA	20	20	50.0	50.0	58.0	58.0	16.5	11.4	0.059808	113405	113654	451-451	0.333333	52.9
trnN-GUU-rpl32_p3	trnN-GUU-rpl32	3	AGCTTTCGAATAGGCATGGG	TAGCTATGGTGTTTTCGGCC	20	20	50.0	50.0	58.0	58.0	16.5	0.0	0.059808	113405	113654	843-843	0.333333	52.9
trnN-GUU-rpl32_p4	trnN-GUU-rpl32	4	TATTGCAATTCCCCGAGTGG	CCAATTGCGGCAAGTACTTG	20	20	50.0	50.0	57.9	58.0	18.7	27.9	0.070081	113405	113654	1026-1026	0.333333	52.8
trnN-GUU-rpl32_p5	trnN-GUU-rpl32	5	TATTGCAATTCCCCGAGTGG	GGGATTTGTTCGGATCGGAA	20	20	50.0	50.0	57.9	58.0	18.7	11.4	0.084015	113405	113654	914-914	0.333333	52.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGCGGAACTGATTGATTGGA	AGGGCAGGCACTAATATTGC	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.501322	115212	115758	1716-1883	1.000000	81.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TGCGGAACTGATTGATTGGA	TAGCGAGCATTCCCTTTTCC	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.526591	115212	115758	1466-1633	1.000000	81.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGCGGAACTGATTGATTGGA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.5	57.9	0.0	0.0	0.612481	115212	115758	1205-1372	1.000000	80.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGCGGAACTGATTGATTGGA	AGATTGTAGGGCAGGCACTA	20	20	45.0	50.0	57.5	58.1	0.0	0.0	0.608629	115212	115758	1723-1890	1.000000	80.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TGCGGAACTGATTGATTGGA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.5	57.8	0.0	0.0	0.673208	115212	115758	1198-1365	1.000000	80.0
ccsA-ndhD_p1	ccsA-ndhD	1	CGAATTCGGCACTTGTAGCT	TCAGTTGACAAAGTACAAGCT	20	21	50.0	38.1	58.6	55.4	20.2	0.0	4.252575	116883	117122	407-427	1.000000	48.7
ccsA-ndhD_p2	ccsA-ndhD	2	ATTCGGCACTTGTAGCTTCT	TCAGTTGACAAAGTACAAGCT	20	21	45.0	38.1	57.2	55.4	0.0	0.0	4.382587	116883	117122	404-424	1.000000	47.7
ccsA-ndhD_p3	ccsA-ndhD	3	ACGAATTCGGCACTTGTAGC	TCAGTTGACAAAGTACAAGCT	20	21	50.0	38.1	58.9	55.4	29.8	0.0	4.536091	116883	117122	408-428	1.000000	46.4
ccsA-ndhD_p4	ccsA-ndhD	4	AATTCGGCACTTGTAGCTTCT	TCAGTTGACAAAGTACAAGCT	21	21	42.9	38.1	57.9	55.4	0.0	0.0	4.671775	116883	117122	405-425	1.000000	45.3
ccsA-ndhD_p5	ccsA-ndhD	5	CGAATTCGGCACTTGTAGCT	ATCAGTTGACAAAGTACAAGCT	20	22	50.0	36.4	58.6	55.9	20.2	0.0	4.700520	116883	117122	408-428	1.000000	45.1
ndhD_p1	ndhD	1	AGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	20	20	40.0	50.0	54.5	58.0	0.0	0.0	3.472947	117123	118540	791-1598	1.000000	57.3
ndhD_p2	ndhD	2	AAGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	21	20	38.1	50.0	55.4	58.0	0.0	0.0	3.630299	117123	118540	792-1599	1.000000	56.1
ndhD_p3	ndhD	3	AGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	20	20	40.0	50.0	54.5	58.2	0.0	0.0	3.672464	117123	118540	796-1603	1.000000	55.7
ndhD_p4	ndhD	4	AGACTTTGCTTTCTGTCTCA	TTTTACCGACAAAAGCCCGT	20	20	40.0	45.0	54.5	58.3	0.0	0.0	3.789035	117123	118540	798-1605	1.000000	54.8
ndhD_p5	ndhD	5	AAGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	21	20	38.1	50.0	55.4	58.2	0.0	0.0	3.829817	117123	118540	797-1604	1.000000	54.5
ndhI-ndhA_p1	ndhI-ndhA	1	GCTCGTATTGTTTGTTGGCC	ACTTCTTCCCAACTTGTTTCAC	20	22	50.0	40.9	58.0	57.0	0.0	0.0	3.006483	121229	121316	168-171	1.000000	60.2
ndhI-ndhA_p2	ndhI-ndhA	2	ACCTTGCAGCTCGTATTGTT	ACTTCTTCCCAACTTGTTTCAC	20	22	45.0	40.9	57.8	57.0	0.0	0.0	3.168675	121229	121316	176-179	1.000000	58.7
ndhI-ndhA_p3	ndhI-ndhA	3	GCTCGTATTGTTTGTTGGCC	CTTCTTCCCAACTTGTTTCACT	20	22	50.0	40.9	58.0	56.7	0.0	0.0	3.277818	121229	121316	167-170	1.000000	58.1
ndhI-ndhA_p4	ndhI-ndhA	4	GCTCGTATTGTTTGTTGGCC	TCTTCCCAACTTGTTTCACT	20	20	50.0	40.0	58.0	54.7	0.0	0.0	3.322644	121229	121316	165-168	1.000000	57.8
ndhI-ndhA_p5	ndhI-ndhA	5	CCCGGTCACCATAATAGGGA	ACTTCTTCCCAACTTGTTTCAC	20	22	55.0	40.9	58.3	57.0	0.0	0.0	3.259127	121229	121316	139-139	0.333333	27.3
ndhA_p1	ndhA	1	GCTCGTATTGTTTGTTGGCC	TGTGAGAGTAGAAGCCCCAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046987	121317	122569	286-2234	1.000000	91.6
ndhA_p2	ndhA	2	GCTCGTATTGTTTGTTGGCC	TGGGGTTTATCAGGACCGAT	20	20	50.0	50.0	58.0	58.1	0.0	4.6	0.135015	121317	122569	618-2567	1.000000	90.9
ndhA_p3	ndhA	3	GCTCGTATTGTTTGTTGGCC	GCTACGAGCTTCTGGAATCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.163429	121317	122569	598-2547	1.000000	90.7
ndhA_p4	ndhA	4	GCTCGTATTGTTTGTTGGCC	GTGTTTTCCCCTGGAGATGG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.183388	121317	122569	230-2178	1.000000	90.5
ndhA_p5	ndhA	5	GCTCGTATTGTTTGTTGGCC	GGAATGGGCATTTTTGGTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.135192	121317	122569	654-1968	0.666667	69.8
ndhH_p1	ndhH	1	CCGCTAGGGCTTGAAGAATT	AGTTGGGTATTCGGGAGTCA	20	20	50.0	50.0	58.0	58.1	0.2	0.0	0.097138	122571	123761	1565-1574	0.666667	68.3
ndhH_p2	ndhH	2	CCGCTAGGGCTTGAAGAATT	TCAGTTGGGTATTCGGGAGT	20	20	50.0	50.0	58.0	58.1	0.2	0.0	0.097138	122571	123761	1567-1576	0.666667	68.3
ndhH_p3	ndhH	3	AATAGATTGAGCAGCAGCCC	AGTTGGGTATTCGGGAGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.074561	122571	123761	1840-1849	0.666667	68.2
ndhH_p4	ndhH	4	AATAGATTGAGCAGCAGCCC	TCAGTTGGGTATTCGGGAGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.074561	122571	123761	1842-1851	0.666667	68.2
ndhH_p5	ndhH	5	GTAATTTGGTCCCATCCGCT	AGTTGGGTATTCGGGAGTCA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.105798	122571	123761	1580-1589	0.666667	68.2
ycf1_p1	ycf1	1	AAGGTCGTTTTCGGGAAGAC	CCAAAATTGGTTAACAGACGGT	20	22	50.0	40.9	58.1	57.4	0.0	8.1	2.702825	127267	127386	197-206	1.000000	62.0
ycf1_p2	ycf1	2	GAAGGTCGTTTTCGGGAAGA	CCAAAATTGGTTAACAGACGGT	20	22	50.0	40.9	57.8	57.4	0.0	8.1	2.737161	127267	127386	198-207	1.000000	61.7
ycf1_p3	ycf1	3	AGAAGGTCGTTTTCGGGAAG	CCAAAATTGGTTAACAGACGGT	20	22	50.0	40.9	57.8	57.4	0.0	8.1	2.743153	127267	127386	199-208	1.000000	61.6
ycf1_p4	ycf1	4	AAAGAAGGTCGTTTTCGGGA	CCAAAATTGGTTAACAGACGGT	20	22	45.0	40.9	57.4	57.4	0.0	8.1	3.203529	127267	127386	201-210	1.000000	57.9
ycf1_p5	ycf1	5	AAGAAGGTCGTTTTCGGGAA	CCAAAATTGGTTAACAGACGGT	20	22	45.0	40.9	57.4	57.4	0.0	8.1	3.203529	127267	127386	200-209	1.000000	57.9
ndhB_p1	ndhB	1	TTCGGAGATTGGATGCAGTT	ATAACCCCAGCCCATTTTGG	20	20	45.0	50.0	57.2	58.1	0.0	0.0	0.909262	142205	144436	2352-2352	1.000000	72.8
ndhB_p2	ndhB	2	TTTCGGAGATTGGATGCAGT	ATAACCCCAGCCCATTTTGG	20	20	45.0	50.0	57.2	58.1	0.0	0.0	0.909262	142205	144436	2353-2353	1.000000	72.8
ndhB_p3	ndhB	3	TTCGGAGATTGGATGCAGTT	GTTCATAACCCCAGCCCATT	20	20	45.0	50.0	57.2	57.8	0.0	0.0	0.946652	142205	144436	2356-2356	1.000000	72.5
ndhB_p4	ndhB	4	TTTCGGAGATTGGATGCAGT	GTTCATAACCCCAGCCCATT	20	20	45.0	50.0	57.2	57.8	0.0	0.0	0.946652	142205	144436	2357-2357	1.000000	72.5
ndhB_p5	ndhB	5	TTCGGAGATTGGATGCAGTT	TTTGGGCGGAACAGATCTAC	20	20	45.0	50.0	57.2	57.6	0.0	0.0	1.184063	142205	144436	2337-2337	1.000000	70.6