Hotspot

clpP

LSC · 69251-71504 · 2254 bp

Back to Habenaria

Pi 0.0248

MarkerSeek score 60.70

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2254
variable_sites: 118
pi: 0.0248
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGTTGGCATGTCGTGTCTAT	CGAACAATAGGCCGATCCAA	50.0	50.0	58.0	58.1	2731-2830 bp	1.0	87.9
clpP_p2	AGTCGCACATACACCCTAGT	CGAACAATAGGCCGATCCAA	50.0	50.0	58.2	58.1	2844-2943 bp	1.0	86.8
clpP_p3	TAATGGTTGGCATGTCGTGT	CGAACAATAGGCCGATCCAA	45.0	50.0	57.8	58.1	2735-2834 bp	1.0	86.5
clpP_p4	GGTTGGCATGTCGTGTCTAT	TTCGAACAATAGGCCGATCC	50.0	50.0	58.0	57.8	2733-2832 bp	1.0	86.4
clpP_p5	GGTTGGCATGTCGTGTCTAT	CCAATACGCAATGGGAGGTT	50.0	50.0	58.0	58.2	2798-2897 bp	1.0	86.4