Hotspot

rpl16-rps3

LSC · 83009-83188 · 180 bp

Back to Habenaria

Pi 0.0617

MarkerSeek score 74.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 24
pi: 0.0617
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CTGAACTCTGCAATGGAGCT	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	57.9	58.0	981-1031 bp	1.0	84.8
rpl16-rps3_p2	TCGTTCCCATAGCTTCTCCA	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.1	58.0	1014-1064 bp	1.0	84.5
rpl16-rps3_p3	AACTCTGCAATGGAGCTTCC	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	58.2	58.0	978-1028 bp	1.0	84.4
rpl16-rps3_p4	GTTTAGGCCTGAACTCTGCA	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	57.8	58.0	989-1039 bp	1.0	84.2
rpl16-rps3_p5	TGTTTAGGCCTGAACTCTGC	AGAGAAGGCAGAGTTCCCTT	50.0	50.0	57.8	58.0	990-1040 bp	1.0	84.2