Hotspot

rpl36-infA

LSC · 79820-79941 · 122 bp

Back to Habenaria

Pi 0.0845

MarkerSeek score 72.30

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 19
pi: 0.0845
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	TCATAACCTCTGCTCGTTGC	AGAATCCGGCGCAGTTTTAT	50.0	45.0	58.0	58.0	795-822 bp	1.0	86.2
rpl36-infA_p2	AAACCACTCCGTGCATTCTT	GGCGGAATGGGGATTGTAAT	45.0	50.0	58.0	58.0	894-934 bp	1.0	85.9
rpl36-infA_p3	TCATAACCTCTGCTCGTTGC	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1124-1164 bp	1.0	85.6
rpl36-infA_p4	GAAACCACTCGACCTCGTAC	GGCGGAATGGGGATTGTAAT	55.0	50.0	58.0	58.0	996-1036 bp	1.0	85.6
rpl36-infA_p5	CAGAAGCCCAAGAAACCACT	GGCGGAATGGGGATTGTAAT	50.0	50.0	58.0	58.0	1008-1025 bp	0.714286	71.3