Hotspot

psaJ-rpl33

LSC · 66328-66896 · 569 bp

Back to Habenaria

Pi 0.0404

MarkerSeek score 78.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 569
variable_sites: 50
pi: 0.0404
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1187-1220 bp	1.0	87.4
psaJ-rpl33_p2	CGAACTGAACTAAGAGCGCT	GGTCAACCTATTCACCCGTC	50.0	55.0	58.0	58.0	1833-1872 bp	1.0	86.9
psaJ-rpl33_p3	ACCCGTGACATTTTGTACCC	GGTCAACCTATTCACCCGTC	50.0	55.0	58.1	58.0	1649-1677 bp	1.0	86.7
psaJ-rpl33_p4	CGAACTGAACTAAGAGCGCT	TAATCGATTCGATCCCCCGA	50.0	50.0	58.0	58.1	1757-1796 bp	1.0	86.6
psaJ-rpl33_p5	CGAACTGAACTAAGAGCGCT	TGAGCTTGAGTTCCGATTGG	50.0	50.0	58.0	57.9	2011-2050 bp	1.0	85.8