Hotspot

psbM-trnD-GUC

LSC · 29112-30056 · 945 bp

Back to Habenaria

Pi 0.0338

MarkerSeek score 78.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 945
variable_sites: 68
pi: 0.0338
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATGAACAGCGCAGTAGCAAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1050-1087 bp	1.0	84.7
psbM-trnD-GUC_p2	TTGGCTGACTGTTTTGACGT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.3	57.9	1102-1139 bp	1.0	84.4
psbM-trnD-GUC_p3	TGAACAGCGCAGTAGCAATA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.6	57.9	1049-1086 bp	1.0	83.8
psbM-trnD-GUC_p4	AAATGAACAGCGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1052-1089 bp	1.0	83.0
psbM-trnD-GUC_p5	AATGAACAGCGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	58.5	57.9	1051-1088 bp	1.0	83.0