Hotspot

atpH-atpI

LSC · 12935-13670 · 736 bp

Back to Habenaria

Pi 0.0416

MarkerSeek score 82.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 736
variable_sites: 67
pi: 0.0416
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.9	58.3	821-833 bp	1.0	87.1
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	50.0	55.0	57.8	58.3	820-832 bp	1.0	86.6
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	GGCTGCGGCTTATATAGGTG	50.0	55.0	58.3	58.3	815-827 bp	1.0	86.2
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.6	58.3	817-829 bp	1.0	85.0
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.6	58.3	816-828 bp	1.0	85.0