Hotspot

trnS-GCU-trnG-GCC

LSC · 7880-8501 · 622 bp

Back to Habenaria

Pi 0.0488

MarkerSeek score 77.70

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 622
variable_sites: 64
pi: 0.0488
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1376-1443 bp	1.0	86.3
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1375-1442 bp	1.0	86.3
trnS-GCU-trnG-GCC_p3	GATCAGGGGAATCGGAGAGA	CATCGTTAGCTTGGAAGGCT	55.0	50.0	58.0	58.0	1445-1498 bp	0.571429	67.5
trnS-GCU-trnG-GCC_p4	ATCAGGGGAATCGGAGAGAG	CATCGTTAGCTTGGAAGGCT	55.0	50.0	58.0	58.0	1444-1497 bp	0.571429	67.5
trnS-GCU-trnG-GCC_p5	GATCAGGGGAATCGGAGAGA	CGTTAGCTTGGAAGGCTAGG	55.0	55.0	58.0	58.1	1442-1495 bp	0.571429	67.3