Hotspot

rps16-trnQ-UUG

LSC · 6056-6454 · 399 bp

Back to Habenaria

Pi 0.0555

MarkerSeek score 81.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 46
pi: 0.0555
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CCGGAATTCGTTGTCTTTGC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1006-1060 bp	1.0	88.9
rps16-trnQ-UUG_p2	CCGGAATTCGTTGTCTTTGC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1593-1652 bp	1.0	87.8
rps16-trnQ-UUG_p3	TCGTTGTCTTTGCTTTTCGC	CTTACAGCAGCTTGCCAAAC	45.0	50.0	57.9	57.9	1586-1589 bp	0.285714	50.4
rps16-trnQ-UUG_p4	CGTTGTCTTTGCTTTTCGCT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	57.9	57.9	1585-1588 bp	0.285714	50.3
rps16-trnQ-UUG_p5	TCGTTGTCTTTGCTTTTCGC	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1003-1007 bp	0.285714	50.2