Hotspot

trnK-UUU-rps16

LSC · 4258-4895 · 638 bp

Back to Habenaria

Pi 0.0524

MarkerSeek score 78.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 72
pi: 0.0524
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAGCCGAGTACTCTACCGTT	TCGATCCCGAAGAGAAGGAA	50.0	50.0	58.2	57.9	851-893 bp	0.571429	66.7
trnK-UUU-rps16_p2	AGATACCCCGGAGACAGAAG	GCAAAGACAACGAATTCCGG	55.0	50.0	57.9	58.0	1953-1954 bp	0.285714	50.7
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1264-1265 bp	0.285714	50.0
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	GCAAAGACAACGAATTCCGG	50.0	50.0	58.2	58.0	1263-1264 bp	0.285714	50.0
trnK-UUU-rps16_p5	AGATACCCCGGAGACAGAAG	TCGATCCCGAAGAGAAGGAA	55.0	50.0	57.9	57.9	1541-1542 bp	0.285714	50.0