Hotspot

rpl22

LSC · 84347-84724 · 378 bp

Back to Gyrotaenia

Pi 0.0159

MarkerSeek score 50.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 378
variable_sites: 6
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TTGTCCCATAATCCCCCACT	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	788 bp	1.0	80.8
rpl22_p2	ATGCAATTCCCTCGACACTC	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.0	58.0	963 bp	1.0	80.6
rpl22_p3	ATCGATACGCCCTGCAATTT	TCCCGAGCGTCTACCATTAT	45.0	50.0	58.0	58.0	1280 bp	1.0	80.4
rpl22_p4	TTGTCCCATAATCCCCCACT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1067-1068 bp	1.0	80.4
rpl22_p5	TTTTCCATCGATACGCCCTG	TCCCGAGCGTCTACCATTAT	50.0	50.0	58.1	58.0	1286 bp	1.0	80.2