Hotspot

ndhE

SSC · 117995-118300 · 306 bp

Back to Gyrotaenia

Pi 0.00654

MarkerSeek score 41.60

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	GGATCGACTCATGGGAGAGA	TCTTTGGACCGTTGGGAATG	55.0	50.0	58.0	58.1	946 bp	1.0	80.1
ndhE_p2	TCCTCTGTTCTTGGAGCAGA	TCTTTGGACCGTTGGGAATG	50.0	50.0	58.0	58.1	1214-1215 bp	1.0	80.0
ndhE_p3	CTTAGCTTTACACCCGTCCC	TCTTTGGACCGTTGGGAATG	55.0	50.0	58.0	58.1	1185-1186 bp	1.0	80.0
ndhE_p4	GATTTGGACCAGAAAAGCGC	TCTTTGGACCGTTGGGAATG	50.0	50.0	58.0	58.1	1437-1439 bp	1.0	79.9
ndhE_p5	GACAGGCGGATTCACATCTT	TCTTTGGACCGTTGGGAATG	50.0	50.0	58.0	58.1	1249-1250 bp	1.0	79.8