Hotspot

ccsA-ndhD

SSC · 115573-115850 · 278 bp

Back to Gyrotaenia

Pi 0.0755

MarkerSeek score 71.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 278
variable_sites: 21
pi: 0.0755
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ATGCAGCTCTTTTATGCGGA	GATTCTGGACCGCGAGAATT	45.0	50.0	58.0	58.1	943-964 bp	1.0	81.3
ccsA-ndhD_p2	TGCAGCTCTTTTATGCGGAT	GATTCTGGACCGCGAGAATT	45.0	50.0	58.0	58.1	942-963 bp	1.0	81.3
ccsA-ndhD_p3	ATGCAGCTCTTTTATGCGGA	TAAAAATGGGCGCGTATGGA	45.0	45.0	58.0	58.0	1548-1569 bp	1.0	80.6
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	TAAAAATGGGCGCGTATGGA	45.0	45.0	58.0	58.0	1547-1568 bp	1.0	80.6
ccsA-ndhD_p5	GCTGGGCGAGATTCAATACA	TAAAAATGGGCGCGTATGGA	50.0	45.0	58.0	58.0	1436-1457 bp	1.0	80.6