Hotspot

rps19-rpl2

IRb · 85026-85144 · 119 bp

Back to Gyrotaenia

Pi 0.0169

MarkerSeek score 63.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 119
variable_sites: 2
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	ATAATGGTAGACGCTCGGGA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	299-300 bp	1.0	80.3
rps19-rpl2_p2	ATAATGGTAGACGCTCGGGA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	265-266 bp	1.0	79.1
rps19-rpl2_p3	ATAATGGTAGACGCTCGGGA	GCAACTCCTTGGGGTTATCC	50.0	55.0	58.0	58.2	273-274 bp	1.0	78.9
rps19-rpl2_p4	ATAATGGTAGACGCTCGGGA	GGGTTATCCTGCACTTGGAA	50.0	50.0	58.0	57.5	262-263 bp	1.0	76.8
rps19-rpl2_p5	ATAATGGTAGACGCTCGGGA	CTTGGGGTTATCCTGCACTT	50.0	50.0	58.0	57.5	266-267 bp	1.0	76.7