Hotspot

trnG-GCC-trnfM-CAU

LSC · 36769-36942 · 174 bp

Back to Gyrotaenia

Pi 0.0119
MarkerSeek score 62.60
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
174
variable_sites
2
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 234-240 bp 1.0 81.9
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 235-241 bp 1.0 81.9
trnG-GCC-trnfM-CAU_p3 AGAAGATGCGGGTTCGATTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 270-276 bp 1.0 81.7
trnG-GCC-trnfM-CAU_p4 GAGAAGATGCGGGTTCGATT GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.1 58.0 271-277 bp 1.0 81.7
trnG-GCC-trnfM-CAU_p5 AGAAGATGCGGGTTCGATTC GCAGTTTGGTAGCTTGCAAG 50.0 50.0 58.1 57.9 263-269 bp 1.0 81.5