Hotspot

atpH-atpI

LSC · 13817-14492 · 676 bp

Back to Gyrotaenia

Pi 0.0074

MarkerSeek score 63.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 676
variable_sites: 5
pi: 0.0074
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.1	57.9	761 bp	1.0	78.6
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	819 bp	1.0	78.5
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	801 bp	1.0	78.4
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	778 bp	1.0	77.7
atpH-atpI_p5	AACGGAAGCAGCAGAAATCA	ACAAGTGGCATTCAAGCTCT	45.0	45.0	58.1	57.7	784 bp	1.0	77.4