Hotspot

psbK-psbI

LSC · 7632-8126 · 495 bp

Back to Gyrotaenia

Pi 0.00877

MarkerSeek score 65.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 4
pi: 0.00877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	GGATTACGTCCCGGATCATT	45.0	50.0	58.1	57.2	592-625 bp	1.0	77.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	587-620 bp	1.0	77.4
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.1	57.1	604-637 bp	1.0	76.6
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	599-632 bp	1.0	76.6
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	584-617 bp	1.0	75.4