Hotspot

trnH-GUG-psbA

LSC · 75-279 · 205 bp

Back to Gyrotaenia

Pi 0.0343

MarkerSeek score 65.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 6
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	245-275 bp	1.0	84.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	259-289 bp	1.0	84.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	258-288 bp	1.0	84.1
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	260-290 bp	1.0	84.1
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCCTCTAGACCTAGCTGCTC	55.0	60.0	58.1	58.4	243-273 bp	1.0	81.8