Hotspot

ndhF-rpl32

SSC · 116848-117858 · 1011 bp

Back to Gyrosphragma

Pi 0.0168

MarkerSeek score 54.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1011
variable_sites: 17
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TAGGTCGCGTGAACCAAAAA	AAAAGGGGGCGTACCATTTT	45.0	45.0	58.0	58.0	1729-1748 bp	1.0	80.4
ndhF-rpl32_p2	TCGCGTGAACCAAAAACCTA	AAAAGGGGGCGTACCATTTT	45.0	45.0	58.0	58.0	1725-1744 bp	1.0	80.4
ndhF-rpl32_p3	TTTTTGACAAGCAGTTGCCG	AAAAGGGGGCGTACCATTTT	45.0	45.0	58.1	58.0	1752-1771 bp	1.0	80.2
ndhF-rpl32_p4	TAGGTCGCGTGAACCAAAAA	ATTGCCACAAGTGAACCGAT	45.0	45.0	58.0	58.1	1804-1823 bp	1.0	79.9
ndhF-rpl32_p5	TCGCGTGAACCAAAAACCTA	ATTGCCACAAGTGAACCGAT	45.0	45.0	58.0	58.1	1800-1819 bp	1.0	79.9