Hotspot

rps11-rpl36

LSC · 84293-84404 · 112 bp

Back to Gyrosphragma

Pi 0.0268

MarkerSeek score 67.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 112
variable_sites: 3
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CATTCCTACGTGAACCCGTT	AAAAATGCCGACTGATCCGT	50.0	45.0	57.9	58.2	232 bp	1.0	78.9
rps11-rpl36_p2	CATTCCTACGTGAACCCGTT	AAGGGCATCCGTTCGTAAAA	50.0	45.0	57.9	57.8	258 bp	1.0	78.7
rps11-rpl36_p3	CATTCCTACGTGAACCCGTT	AAATGCCGACTGATCCGTAG	50.0	50.0	57.9	57.8	230 bp	1.0	78.5
rps11-rpl36_p4	TTTACGTGAACCACTACGCC	AAAAATGCCGACTGATCCGT	50.0	45.0	58.2	58.2	252 bp	1.0	77.8
rps11-rpl36_p5	TTTACGTGAACCACTACGCC	AAGGGCATCCGTTCGTAAAA	50.0	45.0	58.2	57.8	278 bp	1.0	77.6