Hotspot

psbT-pbf1

LSC · 79055-79114 · 60 bp

Back to Gyrosphragma

Pi 0.05

MarkerSeek score 78.80

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 3
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	CTCTTAGTCTCCACTCTAGGGA	GATCCATTCGAGGAACACGG	50.0	55.0	57.2	58.4	171 bp	1.0	55.7
psbT-pbf1_p2	TCTCTTAGTCTCCACTCTAGGG	GATCCATTCGAGGAACACGG	50.0	55.0	57.2	58.4	172 bp	1.0	55.7
psbT-pbf1_p3	CTCTTAGTCTCCACTCTAGGGA	GGCAACCCTCTCAACAACTA	50.0	50.0	57.2	57.4	194 bp	1.0	54.5
psbT-pbf1_p4	TCTCTTAGTCTCCACTCTAGGG	GGCAACCCTCTCAACAACTA	50.0	50.0	57.2	57.4	195 bp	1.0	54.5
psbT-pbf1_p5	CTCTTAGTCTCCACTCTAGGGA	AGATCCATTCGAGGAACACG	50.0	50.0	57.2	57.4	172 bp	1.0	54.5