Hotspot

trnP-UGG-psaJ

LSC · 71146-71595 · 450 bp

Back to Gyrosphragma

Pi 0.0244
MarkerSeek score 69.70
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
450
variable_sites
11
pi
0.0244
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 613-614 bp 1.0 80.6
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 612-613 bp 1.0 80.5
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 624-625 bp 1.0 80.5
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 623-624 bp 1.0 80.5
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG CGCATCCGGGAATAATCGAT 50.0 50.0 58.1 58.0 626-627 bp 1.0 80.5