Hotspot

pafII-cemA

LSC · 65174-65635 · 462 bp

Back to Gyrosphragma

Pi 0.029
MarkerSeek score 61.00
Primer available yes
Label pafII-cemA

Feature Metadata

label_name
pafII-cemA
feature_type
igs
region
LSC
length_bp
462
variable_sites
13
pi
0.029
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
pafII-cemA_p1 TGACTCGGCGAGAAATTGAG AGATCCACCAGGGCAAAAAG 50.0 50.0 58.0 58.1 612-617 bp 1.0 82.9
pafII-cemA_p2 TGAGAATTTGACTCGGCGAG AGATCCACCAGGGCAAAAAG 50.0 50.0 58.0 58.1 620-625 bp 1.0 82.9
pafII-cemA_p3 TGACTCGGCGAGAAATTGAG GAGATCCACCAGGGCAAAAA 50.0 50.0 58.0 58.1 613-618 bp 1.0 82.9
pafII-cemA_p4 TGACTCGGCGAGAAATTGAG GATCCACCAGGGCAAAAAGA 50.0 50.0 58.0 58.1 611-616 bp 1.0 82.9
pafII-cemA_p5 TGAGAATTTGACTCGGCGAG GATCCACCAGGGCAAAAAGA 50.0 50.0 58.0 58.1 619-624 bp 1.0 82.8