Hotspot

rbcL-accD

LSC · 61261-62048 · 788 bp

Back to Gyrosphragma

Pi 0.0166

MarkerSeek score 59.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 13
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTTGTGAAGTGTGGAAGGA	GGCCACGCCTGTATTCTAAT	50.0	50.0	58.0	57.7	906 bp	0.5	57.7
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	GGCCACGCCTGTATTCTAAT	55.0	50.0	57.7	57.7	925 bp	0.5	55.6
rbcL-accD_p3	TGCTTGTGAAGTGTGGAAGG	GGCCACGCCTGTATTCTAAT	50.0	50.0	58.3	57.7	907 bp	0.5	55.4
rbcL-accD_p4	AGCAAGTGGAGTCCTGAACT	GGCCACGCCTGTATTCTAAT	50.0	50.0	58.6	57.7	933 bp	0.5	53.4
rbcL-accD_p5	AGTGGAGTCCTGAACTAGCT	GGCCACGCCTGTATTCTAAT	50.0	50.0	57.4	57.7	929 bp	0.5	53.0