Hotspot

trnE-UUC-trnT-GGU

LSC · 33471-34425 · 955 bp

Back to Gyrosphragma

Pi 0.015

MarkerSeek score 63.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 14
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TCCTTTCGTAGTCCCCTACC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	1081-1099 bp	1.0	81.3
trnE-UUC-trnT-GGU_p2	TCCTTTCGTAGTCCCCTACC	CCGATGACTTACGCCTTACC	55.0	55.0	57.9	58.2	1078-1096 bp	1.0	80.2
trnE-UUC-trnT-GGU_p3	ACTTCCTTTCGTAGTCCCCT	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	1084-1102 bp	1.0	79.9
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACCA	GAACCGATGACTTACGCCTT	50.0	50.0	57.6	58.0	1019-1037 bp	1.0	79.5
trnE-UUC-trnT-GGU_p5	TCCTTTCGTAGTCCCCTACC	TTGAACCGATGACTTACGCC	55.0	50.0	57.9	58.3	1083-1101 bp	1.0	79.1