Hotspot

atpF-atpH

LSC · 14318-14846 · 529 bp

Back to Gyrosphragma

Pi 0.0286

MarkerSeek score 53.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 529
variable_sites: 6
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.40
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCAGTGACCTAAGGAAAC	GGAGGGAAAAATACGAGGCA	55.0	50.0	58.2	57.6	367-685 bp	1.0	80.2
atpF-atpH_p2	GGCCAGTGACCTAAGGAAAC	GGTTGTAGCCTTAGCGCTTT	55.0	50.0	58.2	58.5	298-616 bp	1.0	79.2
atpF-atpH_p3	CAGATGGCCAGTGACCTAAG	GGAGGGAAAAATACGAGGCA	55.0	50.0	57.7	57.6	372-690 bp	1.0	79.0
atpF-atpH_p4	ATGGCCAGTGACCTAAGGAA	GGAGGGAAAAATACGAGGCA	50.0	50.0	58.3	57.6	369-687 bp	1.0	79.0
atpF-atpH_p5	GGCCAGTGACCTAAGGAAAC	ATTGGTTGTAGCCTTAGCGC	55.0	50.0	58.2	58.6	301-619 bp	1.0	78.7