Hotspot

psbK-psbI

LSC · 8528-8930 · 403 bp

Back to Gyrosphragma

Pi 0.015

MarkerSeek score 64.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 6
pi: 0.015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	541-548 bp	1.0	77.7
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	538-545 bp	1.0	75.7
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	539-546 bp	1.0	75.7
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	540-547 bp	1.0	75.7
psbK-psbI_p5	TGTTTGGCAAGCTGCTGTAA	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.6	57.6	542-549 bp	1.0	73.3